Difference between revisions of "Protocols"

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https://genome.duke.edu/cores-and-services/proteomics-and-metabolomics/protocols-reagents
 
https://genome.duke.edu/cores-and-services/proteomics-and-metabolomics/protocols-reagents
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=== PrimeXS Protocols ===
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A collections of protocols used at the Transnational Access sites of the PRIME-XS project.
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These currently include:
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* Determination of protein concentration using the Bradford Assay ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-Bradford-Protein-Assay.pdf/ link])
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* Lysis of cells and tissue samples for LC-MS/MS analysis ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-Cell-Lysis.pdf/ link])
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* NPC HILIC peptide fractionation ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-HILIC-peptide-fractionation/ link])
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* In gel digestion of proteins using trypsin from Coomassie or Silver stained gels ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-In-Gel-Digestion.pdf/ link])
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* In-solution digestion of proteins using various different enzymes ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-In-Solution-Digestion.pdf/ link])
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* In-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-Lys-N-Digestion.pdf/ link])
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* Stable Isotope Labeling (SILAC) by Amino Acids Applied of Bacterial Cell Cultures ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-SILAC-Bacteria.pdf/ link])
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* Enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-TiO2-phosphopeptide-enrichment/ link])
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The complete list of protocols can be found at http://www.primexs.eu/protocols.html.

Latest revision as of 09:37, 20 April 2017

Here we try to present different protocols.

Internal Resources

External Resources

Duke Center for Genomic and Computational Biology

This site provides more than 10 protocols ranging from a basic tryptic digest to phosphopeptide enrichment.

https://genome.duke.edu/cores-and-services/proteomics-and-metabolomics/protocols-reagents

PrimeXS Protocols

A collections of protocols used at the Transnational Access sites of the PRIME-XS project.

These currently include:

  • Determination of protein concentration using the Bradford Assay (link)
  • Lysis of cells and tissue samples for LC-MS/MS analysis (link)
  • NPC HILIC peptide fractionation (link)
  • In gel digestion of proteins using trypsin from Coomassie or Silver stained gels (link)
  • In-solution digestion of proteins using various different enzymes (link)
  • In-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N (link)
  • Stable Isotope Labeling (SILAC) by Amino Acids Applied of Bacterial Cell Cultures (link)
  • Enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis (link)

The complete list of protocols can be found at http://www.primexs.eu/protocols.html.