Difference between revisions of "Protocols"
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https://genome.duke.edu/cores-and-services/proteomics-and-metabolomics/protocols-reagents | https://genome.duke.edu/cores-and-services/proteomics-and-metabolomics/protocols-reagents | ||
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+ | === PrimeXS Protocols === | ||
+ | A collections of protocols used at the Transnational Access sites of the PRIME-XS project. | ||
+ | |||
+ | These currently include: | ||
+ | * Determination of protein concentration using the Bradford Assay ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-Bradford-Protein-Assay.pdf/ link]) | ||
+ | * Lysis of cells and tissue samples for LC-MS/MS analysis ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-Cell-Lysis.pdf/ link]) | ||
+ | * NPC HILIC peptide fractionation ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-HILIC-peptide-fractionation/ link]) | ||
+ | * In gel digestion of proteins using trypsin from Coomassie or Silver stained gels ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-In-Gel-Digestion.pdf/ link]) | ||
+ | * In-solution digestion of proteins using various different enzymes ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-In-Solution-Digestion.pdf/ link]) | ||
+ | * In-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-NPC-Lys-N-Digestion.pdf/ link]) | ||
+ | * Stable Isotope Labeling (SILAC) by Amino Acids Applied of Bacterial Cell Cultures ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-SILAC-Bacteria.pdf/ link]) | ||
+ | * Enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis ([http://www.primexs.eu/protocols/Public-Documents/04---Protocols/PRIME-XS-Protocol-TiO2-phosphopeptide-enrichment/ link]) | ||
+ | The complete list of protocols can be found at http://www.primexs.eu/protocols.html. |
Latest revision as of 10:37, 20 April 2017
Here we try to present different protocols.
Contents
Internal Resources
External Resources
Duke Center for Genomic and Computational Biology
This site provides more than 10 protocols ranging from a basic tryptic digest to phosphopeptide enrichment.
https://genome.duke.edu/cores-and-services/proteomics-and-metabolomics/protocols-reagents
PrimeXS Protocols
A collections of protocols used at the Transnational Access sites of the PRIME-XS project.
These currently include:
- Determination of protein concentration using the Bradford Assay (link)
- Lysis of cells and tissue samples for LC-MS/MS analysis (link)
- NPC HILIC peptide fractionation (link)
- In gel digestion of proteins using trypsin from Coomassie or Silver stained gels (link)
- In-solution digestion of proteins using various different enzymes (link)
- In-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N (link)
- Stable Isotope Labeling (SILAC) by Amino Acids Applied of Bacterial Cell Cultures (link)
- Enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis (link)
The complete list of protocols can be found at http://www.primexs.eu/protocols.html.