From European Proteomics Academy
Revision as of 09:37, 20 April 2017 by Jg (talk | contribs) (Added links to PrimeXS Protocols)
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Here we try to present different protocols.

Internal Resources

External Resources

Duke Center for Genomic and Computational Biology

This site provides more than 10 protocols ranging from a basic tryptic digest to phosphopeptide enrichment.

PrimeXS Protocols

A collections of protocols used at the Transnational Access sites of the PRIME-XS project.

These currently include:

  • Determination of protein concentration using the Bradford Assay (link)
  • Lysis of cells and tissue samples for LC-MS/MS analysis (link)
  • NPC HILIC peptide fractionation (link)
  • In gel digestion of proteins using trypsin from Coomassie or Silver stained gels (link)
  • In-solution digestion of proteins using various different enzymes (link)
  • In-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N (link)
  • Stable Isotope Labeling (SILAC) by Amino Acids Applied of Bacterial Cell Cultures (link)
  • Enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis (link)

The complete list of protocols can be found at