Protocols
Jump to navigation
Jump to search
Here we try to present different protocols.
Contents
Internal Resources
External Resources
Duke Center for Genomic and Computational Biology
This site provides more than 10 protocols ranging from a basic tryptic digest to phosphopeptide enrichment.
https://genome.duke.edu/cores-and-services/proteomics-and-metabolomics/protocols-reagents
PrimeXS Protocols
A collections of protocols used at the Transnational Access sites of the PRIME-XS project.
These currently include:
- Determination of protein concentration using the Bradford Assay (link)
- Lysis of cells and tissue samples for LC-MS/MS analysis (link)
- NPC HILIC peptide fractionation (link)
- In gel digestion of proteins using trypsin from Coomassie or Silver stained gels (link)
- In-solution digestion of proteins using various different enzymes (link)
- In-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N (link)
- Stable Isotope Labeling (SILAC) by Amino Acids Applied of Bacterial Cell Cultures (link)
- Enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis (link)
The complete list of protocols can be found at http://www.primexs.eu/protocols.html.